Fig. 3.
TLR2-, TLR4-, and TLR9-induced effects on costimulatory molecule expression on DCs at 1 and 7 days after injury. Spleen cells were prepared from individual mice at Day 1 or 7 after burn injury. Purified CD11c+ cells were plated at 1 × 105 per well of a 96-well plate and stimulated with 1 μg/ml or BLP, LPS, or CpG ODN 1826. After 18 h incubation, cell suspensions were stained with FITC-labeled anti-CD11c antibody and counterstained with the indicated PE-labeled, anticostimulatory molecule antibodies. The data represent the mean ± sem of n = 3 mice per group of three independent experiments. *, P < 0.05, sham versus burn by ANOVA.