Fig. 6.
The influence of TLR stimulation on DC APC function. DC were purified from the spleens of sham and burn mice and plated at a density of 5 × 104 per well of a 96-well plate with CD4 T cells purified from OTII TCR transgenic mice (2×105 cells) and OVA323–339 peptide (1 μg/ml). LPS, BLP, or CpG ODN (1 μg/ml) were added to the cultures, and after 48 h incubation, supernatants were harvested to test for cytokine production by ELISA. The data represent the mean ± sem of five independent experiments. *, P < 0.05, for sham versus sham; **, P < 0.05, for burn versus burn between TLR agonists as determined by ANOVA. There was no significant difference between sham and burn groups (P<0.05) as determined by ANOVA.