Fig. 7.

Effect of calcineurin inhibitors and MAPK inhibitors on JAM-C induction in activated human T cells, which were cultured and pretreated for 1 h with cyclosporin A (CsA; 1 and 10 μM) or FK-506 (1 μM) or with MAPK inhibitors SP600125 (10 μM), PD98059 (10 μM), and SB203580 (15 μM), and cell culture was continued for 24 h in the absence or presence of PHA (2 μg/ml) or a combination of mAb against CD3 and CD28 (1 μg/ ml), as indicated. Total protein (20 μg) was analyzed by Western blot analysis with antibodies against human JAM-C and GAPDH. Similar results were obtained in three independent experiments. An autoradiogram of a representative experiment is shown. Autoradiographic signals were obtained by chemiluminescence and scanned by videodensitometry. Values ± se represent the fold induction of JAM-C normalized to GAPDH from at least three independent experiments. Statistics, Student’s t-test for paired values: *, significant differences treatment versus control, **, treatment + CsA versus treatment or treatment + FK-506 versus treatment, P ≤ 0.05.