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. 2009 Feb 23;85(5):871–876. doi: 10.1189/jlb.1008635

Fig. 2.

Fig. 2.

Intracellular granzyme and perforin staining of CD107+ and CD107– sorted NK cells. (A) Purified NK cells were incubated with K562 target cells for 3 h at a 4:1 E:T ratio in the presence of mouse anti-human CD107a antibody and sorted as in Figure 1A. CD107+ and CD107– sorted NK cells were then fixed, permeabilized, and stained with 0.2 μg mouse anti-human granzyme (BD PharMingen) or mouse anti-human perforin (BD PharMingen)/million cells. The percentage of NK cells staining positive for granzyme and perforin is shown for each histogram (in bold) along with the mean fluorescence intensity (upper-right corners). Results are representative of three independent experiments.