Figure 6.

MMP and proteasome activities differentially regulate HIV-1-induced alterations of tight junction protein expression. Confluent hCMEC/D3 cells were pretreated with MMP or proteasome inhibitors for 2 h and exposed to HIV-1-infected monocytes for 24 h. JAM-A (A), occludin (B), and ZO-1 (C) expression was determined by Western blotting. Blots are representative images from 3 independent experiments; bar graphs depict quantified results. Results are means ± se. *P < 0.05 vs. control. †P < 0.05 vs. exposure to HIV-1 in absence of inhibitors. iMMP9, MMP9 specific inhibitor; GM, GM 6001 (a general MMP inhibitor); MG, MG-132 (proteasome inhibitor); PSI, proteasome inhibitor I.