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. 2009 May;23(5):1431–1440. doi: 10.1096/fj.08-122903

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Figure 3. — Generation and isolation of heterokaryons. A) Scheme of heterokaryon formation. Human keratinocytes (dsRed) were fused with mouse myotubes (GFP) in differentiation medium, using polyethylene glycol (PEG). B) Images of GFP (green) and dsRed (red) heterokaryon cultures 4 days after fusion. Top panels: characteristic heterokaryon with myotube morphology. Bottom panels: heterokaryons with keratinocyte morphology. C) FACS profiles of human keratinocytes alone (left panel), mouse muscle cells alone (center panel), or heterokaryons (right panel). Sorting gate used for heterokaryon isolation is indicated.