Table 3.

Protein sequence alignment (single letter amino acid code) of regions of four closely related K_2P channels. Shaded letters indicate areas of sequence identity among the four sequences.

	First Extracellular loop															First pore																	Intracellular loop																Second pore
TWIK-1	S	S	V	E	L	P	Y	E	D	L	L	R	Q	E	L	T	T	G	Y	G	H	T	V	P	L	S	D	G	G	K	A	F	T	V	H	V	T	R	R	P	V	L	Y	F	H	I	R	W	Y	F	C	F	I	S	L	S	T	I	G	L	G	D
TWIK-2	A	R	L	E	G	P	H	E	A	R	L	R	A	E	L	T	V	G	Y	G	Y	T	T	P	L	T	D	A	G	K	A	F	S	L	L	L	T	H	V	P	L	S	W	L	S	M	R	W	Y	F	C	F	I	S	L	S	T	I	G	L	G	D
hKCNK7	Q	A	L	E	G	P	P	A	C	R	L	Q	A	E	L	T	T	G	Y	G	H	M	A	P	L	S	P	G	G	K	A	F	L	L	P	V	L	S	R	P	R	A	W	V	A	V	H	W	Y	F	C	F	S	S	L	S	T	I	G	L	E	D
mKCNK7	Q	A	L	E	G	P	P	A	R	H	L	Q	A	Q	V	T	T	G	Y	G	H	M	A	P	L	S	S	G	G	K	A	F	L	L	P	V	F	S	R	P	G	D	W	V	A	I	R	W	Y	F	C	F	G	S	L	S	T	I	G	L	G	D