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. 2009 Apr 15;106(17):7022–7027. doi: 10.1073/pnas.0811785106

Fig. 3.

Fig. 3.

HER2/SV40er tumors develop through a multistep tumorigenesis process in the reconstituted human breast tissue. HER2/SV40er tissue recombinants were subjected to histopathological analysis at various days after implantation. (A) Histopathological analysis of representative breast tissue outgrowth of normal/hyperplasia (Ai–Aiii), DCIS (Aiv–Avi), and invasive carcinoma (Avii–Aix) from tissue recombinants collected at the indicated time points. (Scale bar: 50 μm.) (B) Percent of epithelial area coverage for each lesion type at individual time points. At day 7 after implantation, only normal and hyperplastic outgrowth was observed. DCIS began to emerge at day 10 after implantation, and it became more abundant at days 17 and 25 after implantation. Invasive carcinoma emerged at day 25 after implantation, at which point the lesion grew rapidly, such that by day 47 after implantation, the invasive area took over the majority of the gland area. (C) Distribution of lesion type as a function of age. DCIS outgrowth was most frequently detected between days 17 and 25 after implantation (p.i.), whereas invasive carcinoma was most frequently observed after day 35 after implantation.