Table 5.

Sequence specific primers for reference and target genes for qRT-PCR SCMV experiment

Gene name	Primer sequence (5' - 3')	Annealing temp.
Maize actin 1	For : TCC TGA CAC TGA AGT ACC CGA TTG Rev: CGT TGT AGA AGG TGT GAT GCC AGT T	56.0°C/60.5°C
26S ribosomal RNA gene	For : CAT TCA ATC GGT AGG AGC GAC Rev: GGT CTT CAA CGA GGA ATG CC	60.5°C
Metallothionein-like protein	For : ACT CGG CCC ACA CAG CA Rev: GAG ATG TTG GCG CCG TG	60.5°C
S-adenosylmethionine synthetase 1	For : CCT ATC GGT GTT CGT GGA CA Rev : TGA TCA TGC CGG GCC T	60.5°C
14-3-3-like protein GF14-6	For : GGG AGC CCC CAA ATT TTA CT Rev: AGT GTT TGC TGC TGT CGA ATG	60.5°C
S-adenosylmethionine synthetase 1	For : TCC CAA AAC TGA GCT TGA AGC Rev: GCA GTC TTT GGA TCA AAG CCA	56.0°C
Germin-like protein 4	For : CCC GTC GAA GAA GAA GTC GT Rev: CTT GCT GCT GAC CCC GTA C	56.0°C