Table 2.

Depth Parameters, F, for C2AB or isolated C2B.^†

mutant, Ca2+	C2AB	C2B	mutant, EGTA	C2AB	C2B
L323R1	−2.3±0.2	−2.1±0.1	L323R1	−2.0±0.1	−1.6±0.1
K325R1	−2.0±0.2	−2.9±0.1	K325R1	−2.1±0.2	−2.1±0.1
K326R1	−2.1±0.2	−2.5±0.1	K326R1	−1.9±0.3	−1.8±0.1
K327R1	−2.2±0.2		K327R1	−1.7±0.2
T329R1	−2.1±0.1	−2.1±0.1	T329R1	−1.4±0.4
I330R1	−2.3±0.4		I330R1	−2.2±0.1
K332R1	−2.4±0.5	−2.3±0.1	K332R1	−1.7±0.1	−1.3±0.1
N370R1	−2.3±0.3		N370R1	−2.0±0.2

^†Depth parameters for C2AB are the average of 2 to 3 measurements, and errors represent standard deviations for the data. Previous work has shown that the depth parameters are in the range of approximately −2.0 to −2.5 for labels in the aqueous phase ⁴⁵.