Table 3.

Distances from the R1 side chain to lipid phospholipid phosphates.^†

mutant	ϕ	distance (Å)	Range (Å)
L323R1	−2.0	−5.4	−4.0 < x < −7.3
K325R1	−2.1	−7.3	−4.0 < x < −21.1
K326R1	−1.9	−4.0	−1.3 < x < −10.6
K327R1	−1.7	−2.1	−0.6 < x < −4.0
T329R1	−1.4	0.0	+2.0 < x < −3.0
K332R1	−1.7	−2.1	−1.3 < x < −3.0
N370R1	−2.0	−5.4	−3.0 < x < −10.6

^†Depth parameters and distances for the Ca²⁺-free C2B(C2A) R1 side chains to a plane defined by the lipid phosphates, see Materials and Methods. Negative distances indicate labels that lie on the aqueous side of the membrane-solution interface. The distance range represents the uncertainty in the position of the spin labeled side chain (x) based upon the uncertainty in the experimental measurement of Φ.