Table 2.

Enzyme-linked immunospot (ELISPOT) responses [spot-forming cell (SFC)] of five donors against seven antigenic pox-peptides in the absence or presence of antibodies blocking either human leucocyte antigen (HLA) class II/CD4 or HLA class I/CD8 ligation.

Peptide no.	Peptide	Sequence	Predicted HLA supertype	Assayed KD (nM)	Donors	ELISPOT assay: +Peptideb
						+Isotype	+Anti-CD4	+Anti-CD8
Pox8	A18R255	LSDLKKTIY	A1	3·5	Donor 9	113	89	4a
Pox113	E1L213	FLIDLAFLI	A2	3·0	Donor 19	50	29a	52
Pox114	A47R74	LMDENTYAM	A2	1·0	Donor 19	89	35a	83
Pox116	P1L71	RMIAISAKV	A2	3·4	Donor 13	16	4a	11
					Donor 19	80	31a	70
Pox57	A21R144	KYQSPVNIF	A24	2·2	Donor 21	10	5	3a
Pox17	A10L637	DTRGIFSAY	A26	1·1	Donor 28	93	82	51a
Pox131	I1L48	SEVKFKYVL	B44	4·0	Donor 19	95	19a	93

Each value represents the mean SFC of four individual ELISPOT cultures. Each individual experiment was repeated for two to three times.

^aSFC numbers lower than the isotype control, P < 0·05 (Wilcoxon's rank-sum test).

^bNumber of SFC/10⁵ cells.