Fig. 4.

Detection of macrophage stimulating protein (MSP) (a) and CCL15 (b) proteins in bronchoalveolar cells from patients with pulmonary sarcoidosis; representative result of immunocytochemistry experiments. Positively stained cells were observed in bronchoalveolar lavage cytospin preparations incubated with anti-MSP antibody (a), but not with anti-CCL15 antibody (b) and with an irrelevant antibody (c). MSP was localized to alveolar macrophages; staining was not observed in alveolar lymphocytes. Original magnification ×400.