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Tumour necrosis factor (TNF)-α production in U18666A-treated RAW 264·7 cells. RAW 264·7 cells and peritoneal macrophages were incubated with U18666A (1 µg/ml) for various hours. (a) TNF-α production was determined with enzyme-linked immunosorbent assay. *P < 0·01 versus none (vehicle control). (b) TNF-α mRNA expression was analysed with real-time polymerase chain reaction. *P < 0·01 versus 0 h.
