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Intracellular free cholesterol accumulation in U18666A-treated RAW 264·7 cells. (a) RAW 264·7 cells were incubated with U18666A (1 µg/ml) for various hours in the culture with medium X or medium Y. *P < 0·01 versus none (vehicle control). (b) RAW 264·7 cells were incubated with U18666A (1 µg/ml) for 24 h and stained with filipin. Original magnification × 40.
