Table 2.
Intravital microscopy observations. Diameters (μm) of sinusoids, central venules, portal venules and shunts are expressed as mean ± SEM
| Control (n = 4) | CCl4 4 week (n = 6) | CCl4 8 week (n = 6) | CCl4 10 week (n = 6) | CCl4 12 week (n = 6) | CCl4 16 week (n = 6) | |
|---|---|---|---|---|---|---|
| A: CCl4* | ||||||
| Sinusoidal diameter (μm) | 4.15 ± 0.03 | 3.08 ± 0.02a,b | 3.04 ± 0.03a,b | 3.02 ± 0.03a,b | 3.04 ± 0.03a,b | 2.84 ± 0.03a |
| Central venule diameter (μm) | 8.6 ± 0.4 | 11.9 ± 0.5c,f,g | 10.8 ± 0.3b,d,h | 12.7 ± 0.7e | 14.0 ± 0.5a | 13.6 ± 0.6a |
| Shunt diameter (μm) | Not observed | 15.1 ± 1.0b,i | 14.9 ± 0.6b,j | 15.6 ± 0.7b,k | 18.3 ± 0.8l | 22.2 ± 1.1 |
| Sham-operated 6 week (n = 4) | CBDL 1 week (n = 6) | CBDL 3 week (n = 6) | CBDL 6 week (n = 6) | |
|---|---|---|---|---|
| B: CBDL† | ||||
| Sinusoidal diameter (μm) | 4.35 ± 0.09 | 4.64 ± 0.11 | 4.00 ± 0.13a,c | 3.7 ± 0.05b,c |
| Central venule diameter (μm) | 9.7 ± 1.0 | 10.1 ± 1.2d | 8.1 ± 1.5e | 13.0 ± 2.9 |
| Portal venule diameter (μm) | 11.0 ± 1.1 | 10.5 ± 0.8g | 26.0 ± 2.4f,g | 15.9 ± 3.2 |
| Shunt diameter (μm) | Not observed | Not observed | 22.3 ± 5.1 | 16.2 ± 6.1 |
*
aP < 0.001 vs. control; bP < 0.001 vs. 16 week CCl4; cP = 0.007 vs. control; dP = 0.010 vs. control; eP = 0.002 vs. control; fP = 0.005 vs. 12 w CCl4; gP = 0.034 vs. 16 week CCl4; hP < 0.001 vs. 12 week CCl4; iP = 0.018 vs. 12 week CCl4; jP = 0.003 vs. 12 week CCl4; kP = 0.025 vs. 12 week CCl4; lP = 0.017 vs. 16 week CCl4.
†
aP = 0.001 vs. sham-operated; bP< 0.001 vs. sham-operated; cP = 0.001 vs. CBDL 1 week; dP = 0.05 vs. sham-operated; eP = 0.015 vs. sham-operated; fP = 0.04 vs. sham-operated; gP = 0.02 vs. CBDL 6 week.