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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Exp Parasitol. 2008 Dec 25;122(1):28–36. doi: 10.1016/j.exppara.2008.12.010

Figure 1.

Figure 1

Figure 1

IL-12 is not produced when BMDMϕ are infected with L. amazonensis promastigote forms. (A) BMDMϕs were incubated with late stationary L. amazonensis promastigotes. Culture supernatant fluid was recovered after 24 hours and the presence of IL-12p70 was determined by ELISA. IL-12 concentration in these cultures was compared to supernatant fluid obtained from resting cultures or from cultures of BMDMϕ that were pre-incubated with IFN-γ before stimulation with LPS. (B) mRNA levels of IL-12p40 were determined by real time PCR in infected macrophage samples obtained after 12 hours infection. The threshold cycle (CT) of IL-12p40 for each treatment was normalized to the CT of the reference gene GAPDH for each treatment run in parallel. The fold change in IL-12p40 message compared to uninfected resting macrophages was determined using the delta CT method described in Materials and Methods. For each experiment, the resting BMDMϕ IL-12 message level was arbitrarily set to 100% (i.e. 1) and the fold change from this value for L. amazonensis infected cells was calculated. This allowed comparisons to be made between separate experiments. These results are compiled from 3 experiments.