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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Exp Parasitol. 2008 Dec 25;122(1):28–36. doi: 10.1016/j.exppara.2008.12.010

Figure 3.

Figure 3

Figure 3

Inhibition of Akt activation during infection with L. amazonensis relieves suppression of IL-12p40 at the transcriptional level. (A) BMDMϕ were infected with L. amazonensis in the presence or absence of Akt inhibitor IV and RNA was extracted at 12 hours. IL-12p40 mRNA transcript levels were determined in macrophages by quantitative rtPCR. IL-12p40 CT values were normalized to GAPDH CT values obtained for each treatment. Fold-induction of IL-12p40 was determined by comparing this value to that obtained for uninfected resting BMDMϕ (i.e. resting BMDMϕ fold change = 1). (B) Activity of IL-12 promoter driven luciferase plasmid (pDM4300) in L. amazonensis infected cells. Cells were co-nucleofected with a constitutively expressed Renilla luciferase plasmid. Values are normalized and represented as fold induction over resting cells and are the collation of 3 sets of experimental data. Resting cells were treated with DMSO vehicle. (*) denotes statistical significance, which was determined by the Student’s T test (p<0.05).