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. Author manuscript; available in PMC: 2010 May 1.

Published in final edited form as: Exp Parasitol. 2008 Dec 25;122(1):28–36. doi: 10.1016/j.exppara.2008.12.010

Inhibition of Akt has opposing effects on ERK and p38 phosphorylation. Resting BMDMϕ or those infected with L. amazonensis were incubated with or without Akt inhibitor IV and lysates recovered at various times. Western blot analysis for (A) ERK p44/p42 phosphorylation and (B) p38 phosphorylation are shown. Phospho-p38 levels in the presence of Akt inhibitor is compared to the phospho-p38 without inhibitor blot shown in Figure 4a., which was run in the same experiment (C) Quantitative densitometric analysis for phospho p38 levels was performed with ImageJ software.

Inhibition of Akt has opposing effects on ERK and p38 phosphorylation. Resting BMDMϕ or those infected with L. amazonensis were incubated with or without Akt inhibitor IV and lysates recovered at various times. Western blot analysis for (A) ERK p44/p42 phosphorylation and (B) p38 phosphorylation are shown. Phospho-p38 levels in the presence of Akt inhibitor is compared to the phospho-p38 without inhibitor blot shown in Figure 4a., which was run in the same experiment (C) Quantitative densitometric analysis for phospho p38 levels was performed with ImageJ software.