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. 2009 May 1;5(5):e1000415. doi: 10.1371/journal.ppat.1000415

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Thumbikat et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) 0.1 ug of bovine (lane 1, 3, 5, 7) and human (lane 2, 4, 6, 8) AUM proteins were resolved on 17% SDS-PAGE and transferred onto a nitrocellulose membrane, and detected with monospecific rabbit antisera against Ia (lane 1, 2), Ib (lane 3, 4), II (lane 5, 6), and IIIa (lane 7, 8). All the uroplakins antibodies are monospecific to a single uroplakin band in both bovine and human AUM. The surface expression of uroplakins on PD07i cells were detected using immunofluorescent staining using anti-uroplakins Ia (B), Ib (C), II (D) and IIIa (E) followed with Alexa Fluor 488-conjugated donkey anti-rabbit IgG. Propidium iodide (red) was used to stain nuclei (B–E). Double staining of uroplakins Ia (F) / II (G) / merge (H), and Ib (I) / IIIa (J) / merge (K) showed co-localization of uroplakins on the apical surface of PD07i cells. Arrows mark the co-localization of surface-expressed uroplakins (F–K).