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. 2009 May 8;5(5):e1000467. doi: 10.1371/journal.pgen.1000467

Figure 4. Sch9 deficient mutants metabolize ethanol and accumulate glycerol.

Figure 4

(A) Intracellular glycerol contents of wild type (DBY746) and cells lacking Sch9 were measured on day 1 and day 3. Data represent mean and SEM of 8 cultures analyzed. (B) Glycerol concentration in the medium of wild type and sch9Δ cultures. Data represent mean and SEM of 5–7 cultures analyzed. ** p<0.01, unpaired t-test, two-tailed, sch9Δ vs. WT. (C–D) Glycerol and ethanol concentrations in the medium of wild type (C) and sch9Δ (D) cultures. Data represent mean and SEM of 3–5 cultures analyzed. (E) Nile red staining of neutral lipids of day 1 wild type and sch9Δ mutants. Nile red staining is shown on the right, and phase contrast left. Bar, 10 µm.