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. Author manuscript; available in PMC: 2009 Apr 16.
Published in final edited form as: Science. 2008 Jul 17;321(5889):663–668. doi: 10.1126/science.1157340

Fig. 2.

Fig. 2

Dependence of MEKK1 activation on Ubc13 and IKKγ and association with formation of a multi-component signaling complex. (A) A20 B cells transduced with lentiviruses with no insert or Ubc13 shRNA were stimulated with anti-CD40. Lysates were immunoblotted to detect phosphorylation of MEKK1, JNK, and p38, degradation of IκBα, and expression of Ubc13. (B) Ikkγ-null 1.3E2 B cells and parental 70Z3 cells were stimulated with anti-CD40. At the indicated times, lysates were prepared and analyzed by immunoblotting for MAP3K and MAPK phosphorylation and IκBα degradation. (C, D) Splenic B cells were stimulated with anti-CD40. At the indicated times, cell lysates were prepared and immunoprecipitated (IP) with anti-CD40 (C) or anti-MEKK1 (D) antibodies. The gel-separated immunecomplexes were immunoblotted (IB) with the indicated antibodies.