Skip to main content
. 2009 May 1;5(5):e1000403. doi: 10.1371/journal.ppat.1000403

Figure 4. The I30T mutation in NS2 enhances the efficiency of trans complementation in a genotype-specific manner.

Figure 4

(A) Organization of the chimeric H/J-NS2 replicon expression product, showing the location of the compensatory I30T mutation in the H77-derived sequence [14],[36]. See also Figure 2A. (B) Immunoblot detection of NS2 protein using anti-Myc antibodies. (C,D) Infectious virus yields after transfection of (C) JFH1(SA) or (D) HJ3-5(SA) RNA into VEE replicon cells expressing GFP, or Myc-H/J-NS2 with or without the I30T mutation. Extracellular virus yields were determined three days following transfection. The detection limit of the infectivity assay is indicated by the broken line.