Figure 2.

HGF and EGF stimulate growth in a synergistic manner in the NSCLC cell lines. A549 (A), H1838 (B), and SKMES (C) cells (5000/well) were plated in 96-well plates. After 24 h, wells were treated with HGF 100 ng/ml, EGF 100 ng/ml or both in medium with 10% FBS. At given intervals of time, viable cells were detected by MTT assays and cell viability was plotted as a mean ± SD of three independent experiments. Percentage of cell viability was determined relative to the control that had no additional growth factors. HGF and EGF both induced cell proliferation, and a combination of HGF and EGF had a synergistic effect on cell proliferation in A549 (A), H1838 (B), and SKMES (C)