Figure 4.
Effect of SDF-1μ on Rb cellular localization in HOS-CXCR4 cells. (A) Cytosolic and nuclear extracts (CE and NE, respectively) were obtained from control cells (C) and cells incubated for 4 h in saline solution (S). Immunoblots for Rb show that trophic factors deprivation caused a reduction in the Rb content mainly at the cytosolic level. (B) SDF-1μ prevents changes in Rb expression and subcellular localization induced by long-term serum starvation. Similar data were obtained from two additional experiments. (CE = 25 μg protein/lane; NE = 5 μg protein/lane.)