Skip to main content
. Author manuscript; available in PMC: 2009 Apr 16.
Published in final edited form as: J Neurovirol. 2003 Jun;9(3):300–314. doi: 10.1080/13550280390201010

Figure 6.

Figure 6

Effect of SDF-1μ on E2F-1 in granule neurons and HOS-CXCR4 cells. (A) SDF-1μ prevents the increase in E2F-1 DNA-binding activity induced by K5 (6 h treatment) in cerebellar granule neurons (left panel). The chemokine also inhibits E2F-1 activity in HOS cells (right panel) in the presence or absence of serum (last two lanes). Lanes marked with (−) do not contain nuclear extracts. (B) Western blot analysis in HOS cells: SDF-1μ (20 nM) increases the cytosolic content of E2F-1 and slightly decreases its nuclear level (CE = 25 μg protein/lane; NE = 5 μg protein/lane). (C) Neuronal extracts (20 μg protein/lane) from control and treated cells (6 h) were immunoblotted with E2F-1 antibody: SDF-1μ but not gp120 reduces E2F-1 expression in granule neurons.

HHS Vulnerability Disclosure