Figure 7.
Effect of SDF-1μ, PD0183812, and gp120 on granule neurons survival and Rb phosphorylation. (A) Neuronal death induced by potassium deprivation is inhibited by SDF-1μ (20 nM) and by PD0183812 (0.5 μM), a specific inhibitor of CDK4/6. The CXCR4 antagonist AMD3100 (100 ng/ml) blocked the effect of SDF-1μ. Data from three different experiments are reported in the graph as mean ± SE; * P < .05 versus K25 and ^P < .05 versus K5. (B) gp120IIIB (200 pM) does not inhibit the increase in Rb phosphorylation induced by potassium deprivation (top two panels), whereas increases the level of phosphoRb when neurons are maintained in high potassium (K25, bottom panel). Twenty micrograms of proteins were loaded in each lane.