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. Author manuscript; available in PMC: 2010 Mar 30.
Published in final edited form as: J Neurosci Methods. 2008 Nov 13;178(1):1–9. doi: 10.1016/j.jneumeth.2008.11.003

Figure 3.

Figure 3

A: Double labeling of vagal efferent (green) and vagal afferent (red) innervation of myenteric ganglia was readily achieved by injecting dextran-biotin into the dmnx and dextran-Texas Red into the nodose ganglia. Whole mounts were further processed in the presence of streptavidin Alexa Fluor 488. B: Triple labeling was accomplished using dextran-Texas Red tracing of vagal efferents (red) combined with alpha-synuclein (green) and NOS (blue) immunohistochemical labeling of myenteric neurons in the stomach. Prominent yellow varicosities indicate co-localization of alpha-synuclein with dextran-Texas Red in putative vagal efferent terminals. Scale bars for A = 30 μm; Scale bars for B = 50 μm.