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Expression of FGF2, FGF7 and FGF9 mRNA and FGF2 secretion by NSCLC cell lines. A, quantitative RT-PCR assay for FGF2 and FGF9 mRNAs was performed on the indicated NSCLC cell lines and normalized for GAPDH mRNA levels. B, NSCLC cell lines were cultured for 2 days in serum-free HITES medium. FGF2 secreted into the conditioned medium was measured by ELISA (R&D Systems) and normalized to protein measured in the attached cells.
