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. 2009 Apr 16;106(18):7642–7647. doi: 10.1073/pnas.0902761106

Fig. 1.

Fig. 1.

Generation of Hv1−/− mice. RRN293 ES cells contain a Bay Genomics genetrap vector insertion in the Hvcn1 gene. The transgene serves as a surrogate splice acceptor for wt exon 2 that produces an in-frame chimeric mRNA encoding Hvcn1 exons 1 and 2 followed by β-Geo sequence. (A) Triplex genomic PCR using reverse primers specific for both Hvcn1 and β-Geo amplifies a ≈1.5-kb band from the wt allele (lane 1) and a ≈1.0-kb band from the transgenic allele (lane 3); both alleles are detected in heterozygous wt/RRN293 mice (lane 2). (B) Expression of Hv1 protein (32 kDa) is readily detected in spleen (lanes 1 and 2), BMC (lanes 3 and 4), and PBL (lanes 5 and 6) isolated from wt/wt mice (+/+, lanes 1, 3, and 5) but is absent in RRN293/RRN293 mouse tissue (−/−, lanes 2, 4, and 6).