Table II. Size Exclusion Chromatography and Dynamic Light Scattering*.
| Protein Construct | TraI 1476-1756 | TraI 1476-1630 |
|---|---|---|
| Theoretical MW (kDa) | 31.180 | 16.690 |
| Molar Mass Moments (kDa) | ||
| Mn (% error) | 32.31 (0.11%) | 17.08 (0.5%) |
| Mw (% error) | 32.32 (0.11%) | 17.35 (0.5%) |
| Mz (% error) | 32.33 (0.25%) | 17.61 (0.5%) |
| Polydispersity | ||
| Mw/Mn (% error) | 1.000 (0.2%) | 1.016 (0.7%) |
| Mz/Mn (% error) | 1.001 (0.3%) | 1.041 91.1%) |
*
Weight average molar mass defined as Mw=Σ(ci·Mi)/ Σci, Number average molar mass defined as Mn = Σci / Σ(ci/Mi), Z-average molar mass defined as Mz = Σ(ci·Mi)2/ Σ(ci·Mi).
Polydispersity of the sample equals one only when the sample has homogenous molecular mass (i.e. one oligomeric state and independent of averaging method).