Table 1.

Checkerboard Assay. Varying concentrations of ATP or C5a were loaded into the top, bottom, or both wells of the Boyden Chamber.

		Top Well [ATP] (μM)
		0	30	300
Bottom Well [ATP] (μM)	0	100±5	166±15	254±17**
	30	450±34**	417±18**	337±27**
	300	675 ±54**	859±86**	582±45**
		Top Well [C5a] (nM)
		0	3	30
Bottom Well [C5a] (nM)	0	100±5	113±13	98±11
	3	387±52**	138±20	89±11
	30	404±39**	285±46**	139±29

Numbers shown represent migration measured on the bottom side of the filter, expressed as a percentage of basal migration ±SEM. n=9 (i.e. 3 independent experiments performed in triplicate);

^*p<0.05,

^**p<0.01 compared to basal (ANOVA followed by Dunnett’s post test)