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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Invest Dermatol. 2008 Nov 13;129(5):1174–1184. doi: 10.1038/jid.2008.349

Figure 2. High-level expression of gD following CJ9-gD infection of Vero cells.

Figure 2

A.Vero cells were seeded at 1 × 106 cells per 60-mm dish. At 23 h after seeding, cells in duplicate were either mock-infected or infected with CJ83193, CJ9-lacZ, or CJ9-gD at an MOI of 5 PFU/cell. Infected cell extracts were prepared at 24 h post-infection. B. Vero cells were infected with the wild-type HSV-1 strain KOS, or CJ9-gD at an MOI of 20 PFU/cell. Infected cell extracts were prepared at 4 h and 6 h post-infection. Proteins in infected cell extracts were resolved on SDS-PAGE, followed by immunoblotting with a monoclonal antibody specific for ICP4 or a polyclonal antibody specific for gD.