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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Invest Dermatol. 2008 Nov 13;129(5):1174–1184. doi: 10.1038/jid.2008.349

Figure 3. Trans-dominant-negative effect of CJ9-gD on replication of wild-type herpes simplex virus type 1 (HSV-1).

Figure 3

Vero cells were seeded at 7.5 × 105 per 60-mm dish. A. At 24 h post-seeding, cells in triplicate were infected with either wild-type HSV-1 strain KOS at an MOI of 1 PFU/cell, KOS at an MOI of 1 PFU/cell and CJ9-gD at an MOI of 3 PFU/cell, or KOS at an MOI of 1 PFU/cell and CJ9-gD at an MOI of 5 PFU/cell. B. Vero cells were infected with wild-type HSV-1 strain KOS at an MOI of 0.5 PFU/cell. At 1 h post-infection, cells were either mock-superinfected or superinfected with CJ9-gD, or an ICP4 deletion mutant, D2, at an MOI of 3 PFU/cell. Infected cells were harvested at 18 h post-wild-type HSV-1 infection and viral titers were determined on Vero cell monolayers. Viral titers are expressed as the mean +/- SD. Numbers on the top of the graph indicate the fold of reduction in wild-type virus yield between single infection and co-infection.