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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Invest Dermatol. 2008 Nov 13;129(5):1174–1184. doi: 10.1038/jid.2008.349

Figure 7. Prevention of herpetic keratitis and establishment of latent infection by challenge virus in immunized mice.

Figure 7

A. Female BALB/c mice were randomly divided into 4 groups of 8 mice each and immunized with mock-infected cell lysate, CJ83193, CJ9-lacZ, or CJ9-gD as described. Four weeks after initial immunization, both eyes of the mice were challenged with HSV-1 strain mP following corneal scarification. Individual eyes were scored for severity of keratitis during a 30-day follow-up period. The indicated values represent the mean score ± SEM of all eyes from each group of mice. B. Mice described in panel A and from a similar experiment in which mice were immunized with either mock-infected cell lysate (n = 10), CJ83193 (n = 8), CJ9-lacZ (n = 8), or CJ9-gD (n = 10) were euthanized 30 days after challenge. Trigeminal ganglia of mice from different groups were processed and co-cultivated individually onto Vero cell monolayers. After 5 days of co-cultivation, explants were harvested, processed, and assayed on Vero cell monolayers for the presence of infectious viruses.