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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Glia. 2009 Jun;57(8):828–834. doi: 10.1002/glia.20809

Fig. 6. Astrocytes did not express CaMKIV.

Fig. 6

CaMKIV mRNA and protein expression was analyzed in neurons (N) and astrocytes (A). (A) CaMKIV RNA expression determined by Q-PCR, relative to the signal obtained when reverse transcriptase was omitted (gDNA). Error bars show mean ± sem, n = 4 experiments. (B) Western blot analysis of CaMKIV expression, which was detected in neurons (left lane) but not in astrocytes (right lane). Neuronal bands at 61 and 63 kDa corresponded to the α- and β-isoforms of CaMKIV. Blots were stripped and reprobed for β-actin. CaMKIV protein expression in astrocytes, normalized to β-actin expression, was 3.2 ± 2.4% of that in neurons (n = 3 experiments).