Figure 3. DACH1 regulation of AR activity.

Androgen-response luciferase reporter genes were assessed for DHT-mediated activity in transient expression studies. Cells were co-transfected with expression vectors encoding DACH1, mutant DACH1 (ΔDS) or Ski. Cells were treated with AR agonist (either R1881-10nM or DHT-10nM). Luciferase reporter gene assays were conducted in distinct cell lines as indicated (A; B; CV1) (C; DU145) (D; LNCaP). Data are mean ± SEM of N≥5 separate transfections.