Skip to main content
. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Cancer Res. 2009 Apr 7;69(8):3347–3355. doi: 10.1158/0008-5472.CAN-08-3821

Figure 4. DACH1 associates with the AR and is co-recruited to an endogenous ARE.

Figure 4

(A) Schematic representation of the human PSA promoter indicating the ARE (I-III) (B) ChIP analysis of LNCaP cells stably transduced with a Doxy-regulated DACH1 expression vector. Cells were treated for 12 hours with either DHT (10nM), Casodex (CDX, 15μM), or vehicle control. ChIP assays were conducted with the antibodies indicated. (C) immunoprecipation-western blot assay in LNCaP cell. (D) Association of DACH1 and AR in mammalian two-hybridization assay.