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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Cancer Res. 2009 Apr 7;69(8):3347–3355. doi: 10.1158/0008-5472.CAN-08-3821

Figure 5. DACH1 inhibition of AR reporter gene activity via the AR acetylation cite and overcome by p300.

Figure 5

(A). Luciferase reporter gene assays were conducted using the AR-responsive PSA-Luc reporter gene. Cells were co-transfected with the AR wt or AR mutants and DACH1 DS domain as indicated. The AR K630 site is acetylated in response to ligand. ARK630Q represents a gain-of-function mutant, ARK630A, an acetylation dead mutant. (B) AR wt or carboxyl terminal ligand binding mutants were assessed for DACH1 repression. (C) p300 and (D) SRC-1 enhanced AR activation and overcome DACH1 repression. Data are mean ± SEM of N≥5 separate transfections.