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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Cancer Res. 2009 Apr 7;69(8):3689–3694. doi: 10.1158/0008-5472.CAN-09-0024

Figure 2.

Figure 2

Hormonal and molecular characterization of mutant strains. A, TSH and T4 levels in 6 to12-week old mice (n=8-15), shown as box plots. The ends of the box are the 25th and 75th quantiles. The line across the box identifies the median value. Whiskers extend to the outermost data point. (*) P<0.05, Student t-test. B, Western blot analysis of MEK and ERK activation in pooled thyroids, normalized to β-actin. C, qRT-PCR analysis of Mek1 and Mek2 thyroid expression in mice of the four different genotypes. (*) P<0.05; (**) P>0.05. D, immunohistochemical detection of ERK1/2 phosphorylation, showing the cooperative effect of PI3K and Kras activation. Original magnification, 200×.