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. 2009 Apr 23;4(4):e5301. doi: 10.1371/journal.pone.0005301

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A. H460 cells were treated, fixed and permeabilized. Immunostaining with p65 antibody shows a cytosolic distribution of the protein in control cells and a clear nuclear localization when treated with 10 μM CPT. B. Effect of the inhibitor of NF-κB on cell sensitivity to CPT. H460 viability was tested by cytometric analysis of PI incorporation. * P<0.05 between treatments and their controls C. Effect of the presence of the inhibitor Bay 11-7085 (BAY) on the CPT dependent increase in CoQ levels. Results are the mean±SD of three independent experiments. * P<0.05 between control and the CPT -treated group. D. Relative expression of COQ7 gene measured by real time PCR. Results are normalized on the basis of the expression of the housekeeping actin gene. Results are the mean±SD of three independent experiments; * P<0.05.