Fig. 1.

General experimental design. The experiments were aimed at initially analyzing the sensitivity of mebrofenin handling to CCl₄-induced liver injury. (a) CCl₄ was administered in doses of 0.25, 0.5, and 1 ml/kg 1 day before analysis of liver tests, histology and mebrofenin handling. (b) Plan for testing whether acute inflammation induced by CCl₄ resulted in perturbations of mebrofenin handling in the long term, up to 28 days. These studies used groups of six rats per time-point. (c) Treatment of animals with LPS and cytokines followed by mebrofenin analysis after various intervals. LPS, lipopolysaccharide.