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. 2009 Mar 10;100(7):1120–1127. doi: 10.1038/sj.bjc.6604980

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Copyright © 2009 Cancer Research UK

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BCRP and pAkt expression in Caco-2 and WiDr HF- and LF-adapted cells and MCF-7/MR cells. (A) BCRP protein expression was determined by western blot analysis in Caco-2 and WiDr HF- and LF-adapted cells and MCF-7/MR cells. Per lane 15 μg (Caco-2), 30 μg (WiDr) or 1 μg (MCF-7/MR) of protein was loaded. As a loading control, β-actin levels are indicated. (B) BCRP mRNA levels assessed by real-time LC-PCR were determined in Caco-2 and WiDr HF- and LF-adapted cells. Shown are the ratios between BCRP and the housekeeping gene, β-actin, presented as percent (%) of the HF cells. Bars represent the arithmetic means±s.e.m. of two independent experiments performed in duplicate. (C) pAkt protein expression was determined by western blot analysis in Caco-2 and WiDr HF- and LF-adapted cells. Per lane 30 μg of protein was loaded. As a loading control, β-actin levels are indicated. Breast cancer resistance protein in Caco-2, data from Lemos et al (2008b).