Figure 3.

Cancer cells but not normal cells incubated in Surv-WT-conditioned medium take up the Flag-HA-survivin fusion protein. (A) HeLa, Capan1, Capan2, MCF-7, Panc1, PC3, SaOS2, U2OS, HCC, and HBMS cells were cultured with control- or Surv-WT-conditioned medium for 24 h. Cells were fixed and stained with antibodies to HA, magnification × 1000. (B) Western blot analysis using antibodies to survivin shows the retarded ∼18 kDa band of the Flag-HA-survivin fusion protein only in those lysates from the cells grown in conditioned medium. A third band, labelled ^*, often appears after probing with the antibodies survivin whose identity is yet unknown to us. Molecular weight markers in kilodaltons (kDa) are shown on the left.