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. 2009 Mar 17;100(7):1073–1086. doi: 10.1038/sj.bjc.6604978

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Copyright © 2009 Cancer Research UK

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Recombinant survivin protein is unable to cross the HeLa cell membrane. (A) Detection of cellular recombinant proteins. These images show a portion of a 96-well plate. The right panel is a composite image showing the fluorescence in both the 700- and 800-nm detection channels. Duplicate rows of microplate wells loaded with 0.001, 0.01, or 1.0 μg of protein are shown. The middle panel shows detection of endogenous survivin protein, which was used as a loading control. The left image shows detection of increasing amounts of recombinant survivin (BIRC5), transferrin, and CTB. (B) Quantification of fluorescence. Recombinant protein signal has been normalised using the endogenous survivin protein signal from each well to correct for well-to-well variation in cell number. Recombinant protein (rProtein).