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. 2009 Mar 10;100(7):1095–1102. doi: 10.1038/sj.bjc.6604970

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Copyright © 2009 Cancer Research UK

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EphA1 mRNA and protein expression in colorectal cancer (CRC) cell lines. (A) EphA1 transcript levels normalised to 1000 copies of β-actin quantitated by qPCR. (B) EphA1 protein expression in LIM1215 and LOVO cell lines determined through immunoprecipitation followed by western blot analysis using an in-house rabbit anti-EphA1 antibody. (C) EphA1 protein levels determined by flow cytometry analysis. Fluorescence resulting from EphA1 protein expression (shaded) is shown relative to the secondary-only control cells. The median fluorescence value is shown.