Figure 1. The selective agonist 8-pCPT activates Epac, but not PKA.

A, hippocampal slices were exposed to forskolin (20 μm) or 8-pCPT (200 μm) for 15 min and the CA1 region was dissected to measure phospho-CREB accumulation, using Western immunoblot analysis. Similar experiments were performed in triplicate. B, quantification of western immunoblots of phospho-CREB accumulation. C, pKA activity was measured from hippocampal CA1 region extracts, in the absence of and after 15 min exposure of the hippocampal slices to H89 (2 μm), forskolin (20 μm), forskolin + H89, or 8-pCPT (200 μm), using a colorimetric kit (EKS-390 A, Stressgen). The OD values were normalized to control value. In panels B and C, each bar represents the mean ±s.e.m. of 3 experiments. Asterisks indicate values that are significantly different from control.