Figure 2. Epac-mediated depression of synaptic transmission.

The initial slope of CA1 field EPSPs (inserts) recorded from hippocampal slices was measured before, during (horizontal bar) and after application of 8-pCPT (200 μm). A 45 min pretreatment of the slices with BFA (20 μm, n= 5, A), Rap1 inhibitor GGTI (10 μm, n= 5, C), p38-MAPK inhibitor SB203580 (5 μm, n= 5, D), or PKCε inhibitory peptide TAT-εV1-2 (5 μm; n= 5; F), but not PKA inhibitor H89 (2 μm, n= 5, B), or MEK1 inhibitor PD98059 (25 μm, for 1 h before recording, n= 5, E) inhibited the 8-pCPT-induced LTD. The control curve in all panels was obtained from 20 pooled interleave experiments.