G6Pase (A), PEPCK (B), PDK4 (C), PGC-1α (D), Angptl-4 (E), IRS-2 (F), IRS-1 (G), Fasn (H), COXI (I), and CPT1a (J) mRNA content in the liver of sedentary (sed) or exercised (run) mice. Values are shown as arbitrary units (n= 8, mean ±s.e.m.). K, 150 μg protein of liver and white gastrocnemius muscle extracts were separated by 7.5% SDS-PAGE and immunoblotted with antiphospho-Thr-172 antibodies and reprobed with mixed anti-AMPK α1 +α2 antibodies. Shown are representative blots with two animals of each group.