Figure 8. RT-PCR analysis of GABAergic marker expression in whole P14 rat carotid bodies.

Products were run on 2% agarose gel stained with ethidium bromide and viewed under UV illumination. A, expression of GABA_A receptor subunits. B, expression of GABA transporter (GAT) isoforms. C, expression of GABA biosynthetic enzyme isoforms. Ladder band sizes are given in base pairs. Lamin (A/C) housekeeping gene was used as a positive control. No RT lane refers to negative control reactions in which water replaced Superscript III reverse transcriptase. All band identities were verified by sequencing (MOBIX, McMaster University, Canada).